monocytic leukemia cell line thp1  (ATCC)

Journal: Nature Communications

Article Title: Extracellular vesicles derived EBV tegument protein BRRF2 suppresses cGAS phase separation to promote anti-viral innate immune evasion

doi: 10.1038/s41467-025-64037-2

Figure Lengend Snippet: A The box plot shows the differences in the immune and stromal cell compositions between the two groups of tumor samples with different BRRF2 expression levels based on the RNA-seq data. p values were determined via an unpaired two-side t test. Center line, median (50th percentile); box bounds, 25 and 75th percentiles (interquartile range, IQR); whiskers, extend from the bounds to the smallest or largest value within 1.5 × IQR of the bound; minima and maxima, the end points of the whiskers. B Representative images of panCK (green), BRRF2 (yellow) and CD68 (orange) staining in NPC patients’ tumor tissues. Scale bars, 5 μm. C The average distance from BRRF2 + or BRRF2 - tumor cells to CD68 + cells was measured from the IHC images. Each dot represents a patient’s tumor tissue. The experiment was performed as three independent biological replicates. ** p < 0.01 according to a two-sided paired t test. D HPMs and THP1 cells were incubated with HEK293T-induced EVs carrying mCherry-BRRF2 for 4 h, and mCherry-BRRF2 was internalized into the cells. E HPMs and THP1 cells were incubated with HEK293T-induced EVs carrying mCherry-BRRF2 for 4 h. mCherry signals were detected by flow cytometry. The median fluorescence intensity (MFI) is shown. (Means ± SEMs of n = 3 experiments. **** p < 0.0001 according to a two-sided unpaired t test). F EVs from the HEK293T-Flag-BRRF2 cell line inhibited cGAS-STING activation induced by HT-DNA in THP1 and HPM cells. THP1 cells and HPMs were incubated with EVs from the HEK293T-Flag-BRRF2 cell line for 2 h and then transfected with 2 μg of HT-DNA for 6 h. Cell lysates were collected for immunoblotting with the indicated antibodies. The experiment was performed as three independent biological replicates. Representative images was shown. Source data are provided as a file. G After an incubation with EVs and transfection with HT-DNA, total mRNAs were extracted from THP1 cells for a qPCR analysis of the expression of the IFNβ, RANTES and IL6 mRNAs. The error bars are derived from triplicate experiments. The data are plotted as the means ± SEMs. ns indicates not significant. **** p < 0.0001 according to two-way ANOVA followed by Sidak’s multiple comparisons test.

Article Snippet: The human acute monocytic leukemia cell line THP1 (ATCC, #TIB202), the murine breast cancer cell line EMT6 (ATCC, #CRL-2755), and the human NPC cell lines HONE1, CNE2, HK1 (provided from Dr. Sai-Wah Tsao, University of Hong Kong), and Raji (ATCC, #CCL-86), and latent EBV-infected CNE2 (EBV + CNE2) cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37 °C with 5% CO 2 .

Techniques: Expressing, RNA Sequencing, Staining, Incubation, Flow Cytometry, Fluorescence, Activation Assay, Transfection, Western Blot, Derivative Assay

Journal: Frontiers in Oncology

Article Title: Interrogation of macrophage-related prognostic signatures reveals a potential immune-mediated therapy strategy by histone deacetylase inhibition in glioma

doi: 10.3389/fonc.2025.1554845

Figure Lengend Snippet: Prediction and validation of the potential anticancer drugs. (A) Correlation between CD163 expression and risk score in the TCGA database (p <0.0001). (B) Differences in mRNA expression of various prognostic genes and CD163 in the indirect co-culture model (*p <0.05; * *p <0.01; * * *p <0.001; * * * *p <0.0001). (C) Expression of CD163 and risk score in the indirect co-culture model. (D) Potential anticancer drugs were predicted using signature genes and a risk score. (E) The U87MG (RP) was cocultured with the PMA-induced adherent THP1 (GP), and the treatment groups were then treated with Vorinostat. (F) Analysis of growth of U87MG and U87MG cocultured with PMA-induced adherent THP1 before and after Vorinostat treatment. (G) Expression of macrophage-related immune checkpoints of THP1 before and after the treatment with Vorinostat in the U87MG-THP1 indirect co-culture model (*p <0.05; * *p <0.01; * * *p <0.001; * * * *p <0.0001). (H) The expression of Cd163 in RAW264.7 cells at the protein level, with GL261 conditioned medium and Vorinostat as variables.

Article Snippet: Human glioma cell lines A172 and U87MG, human monocytic leukemia cell line THP1, mouse macrophages cell line RAW264.7 were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Biomarker Discovery, Expressing, Co-Culture Assay